Tissue Clearing Kit (Hydrophilic)

Tissue Clearing Kit (Hydrophilic)

Product description: 

The product can be used for clearing of common animal tissues. Tissue clearing is achieved through delipidation and refractive index matching by immersing the tissue sequentially in the hydrophilic chemicals contained in this product following the instruction.

Product features: 

1. The product can be used to clear both intact and sectioned tissues. The cleared tissues are suitable for imaging with common microscopes, such as light sheet microscope and confocal microscope, etc..

2. The product has a high clearing efficiency. For instance, it usually takes 5-7 days to clear an adult mouse brain and ~2 minutes to clear a ~200 μm thick mouse brain slice. 

3. The product can be used to clear tissues with and without endogenous fluorophores. Genetically encoded fluorophores can be preserved with high efficiency after clearing. Fluorescent staining can be applied before or after delipidation following the instruction.

4. The cleared tissue has a high mechanical strength and preserve the cellular and subcellular structures effectively.

5. The product uses low toxicity hydrophilic chemicals for tissue clearing.

A mouse CNS before and after tissue clearing with the kit:

3D Image of the cleared mouse CNS

       Comparison of tissue clearing efficiency on P7 rabbit brains:

Imaging Case：

Thy1-GFP mouse brain

Anti-TH labeled mouse heart

Anti-TH labeled mouse liver

Experimental operation video：

Experimental operation video：

相关彩页：

组织透明化试剂盒（亲水型）-宣传彩页.pdf

亲水性组织透明化试剂盒-使用说明书_2024.pdf

组织切片样本透明化试剂盒-使用说明书.pdf

系列货物清单：

Imaging Case Video：

Thy1-GFP成年小鼠完整中枢神经系统

采用锘海亲水型透明化试剂盒制样

分辨率：2μm×2μm×5μm

小鼠肺-自发荧光-3.2X

采用锘海亲水型组织透明化试剂盒

分辨率：4 μm X 4 μm X 10.0 μm，成像尺寸：12.6 mm X 14.0 mm X 5.7 mm，拍摄时长：30 mins，放大倍数：3.2X

不同荧光染色的小鼠全脑

采用锘海亲水型透明化试剂盒、锘海增强型透明化试剂盒

SM22:大血管 Lctin:微血管 TH: 多巴胺能神经元 Syto&P1: 细胞核

分辨率：4μmX4μmx10μm

了解更多透明化试剂盒相关信息

请联系021-37827858 或 13818273779（微信同号）