Tissue Expansion Kit

Tissue Expansion Kit

Product description: 

The product immobilizes biomacromolecules to a hydro-gel. Tissue expansion is further achieved through isotropicexpansion of the hydro-gel in water, which improves imaging resolution with the same imaging method.

Product features:

1. The product can be used for expansion of both intact and sectioned tissues. 

2. The expanded tissues are suitable for imaging with common microscopes, such as light sheet microscope and confocal microscope, etc..

3. The product has a high tissue expansion efficiency. For instance, the expansion of a whole adult mouse brain only takes 9-10 days.

4. The product can be used to expand tissues with and without endogenous fluorophores. Genetically encoded fluorophores can be preserved with high efficiency after expansion. Fluorescent staining can be applied before delipidation, after delipidation, or after expansion following the instruction.

5. The expanded tissue has a high mechanical strength and preserve the cellular and subcellular structures effectively.

6. The expansion ratio can be adjusted between 3 and 4 following the instruction.

7. The product uses low toxicity chemicals for tissue expansion.

The expansion process of a mouse brain：

The expansion of various mouse organs：

Imaging Case ：

Thy1-GFP mouse brain neuron 

4X  expansion

Resolution：0.5×0.5×1.25 μm3

Thy1-GFP mouse brain neuron 

4X  expansion

Resolution ：70×70×200 nm³

Green: ChAT-GFP mouse spinal cord motor neuron

Purple: PV-tdTomato mouse sensory neuron

4X  expansion

Resolution ：100×100×250 nm³

Experimental operation video：

Experimental operation video：

相关彩页：

组织膨胀试剂盒（压缩版）.pdf

组织膨胀试剂盒说明书（压缩版）.pdf

组织切片膨胀试剂盒-使用说明书.pdf

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