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Tiling light sheet 3D imaging

Nuohai LS18 tiling light sheet microscope is a versatile tilling light sheet micro scope with semi-automatic calibration capability.It can quickly tile multiple small and thin light sheets (up to 1μm) for illumination under the same field of view(FOV) which stands for higher spatial resolution under same condition.Nuohai LS18 tiling light sheet microscope is suitable for multichannel rapid 3D imaging of tissue samples transparentized by all types of tissue clearing methods, which can reach 1 μm horizontally and 3 μm vertically imaging spatial resolution as needed.

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小鼠脊髓(ChAT-GFP+Lectin)

采用锘海组织透明化试剂盒制备样品,LS18光片显微镜6.3X放大倍率成像,Lectin红色,ChAT-GFP绿色


Thy1-GFP-line M 小鼠大脑皮层椎体神经元三维成



E8小鼠胚胎膨胀样品(细胞核&Lectin阳性细胞)

采用锘海组织膨胀试剂盒和LS18 光片显微镜6.3X倍率成像


小鼠脑海马血管提取

使用锘海自主透明化方法,平铺光片显微镜4X快扫成像


小鼠整脑成像及三维重构

使用LS18平铺光片显微镜仅需10秒


Mice intestine

Cleared and labeled with our pipeline, imaged with LS18 at 4X


SM22 staining reveals blood vessels in a mouse epididymis fat

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X


Tyrosine Hydroxylase staining reveals sympathetic nerve s in a mouse epididymis fat

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X


Endogenous Thy1-driven EGFP expression visualized throughout a P15 mouse entire CNS (whole brain and spinal cord).

Cleared with our pipeline, imaged with LS18 at 4x.


Mouse spleen labeled with Tyrosine Hydroxylase

Cleared and labeled with our pipeline, imaged with LS18 at 23x.


小鼠脊髓透明化膨胀样本


3D rendering of an expanded adult ChAT-eGFP mouse lumbar spinal cord section


3D rendering of the cleared spinal cords of four ChAT-eGFP mice at ages of P1, P7, P28 and P56


3D rendering of a cleared adult WT mouse spinal cord with sparsely labeled ReST axons


3D rendering of an expanded adult ChAT-eGFP mouse lumbar spinal cord section


3D rendering of a cleared adult WT mouse spinal cord with sparsely labeled FL-spinal CST axons


3D rendering of a cleared adult WT mouse spinal cord with sparsely labeled RST axons


3D rendering of a cleared adult WT mouse spinal cord with sparsely labeled sensory neurons.



3D rendering of an expanded cervical spinal cord section of an adult ChAT-eGFP mouse with sparsely labeled ReST axons. 


3D rendering of an expanded lumbar spinal cord section of an adult ChAT-eGFP mouse with sparsely labeled L1 DRG proprioceptive sensory neurons


3D rendering of an expanded cervical spinal cord section of an adult ChAT-eGFP mouse with sparsely labeled ReST axons. 


3D rendering of a cleared adult ChAT-eGFP mouse cervical spinal cord section


3D rendering of an expanded cervical spinal cord section of an adult ChAT-eGFP mouse with sparsely labeled FL-spinal CST axons


3D renderings of expanded cervical and lumbar spinal cord sections of an adult ChATeGFP mouse with sparsely labeled HL-spinal CST axons.


PI (Propidium Iodide) staining of an entire mouse brain hemisphere.

Cleared and labeled with our pipeline, imaged with LS18 at 3.2x



3D rendering of a cleared adult Thy1-eGFP mouse spinal cord


      3D rendering of an expanded adult Thy1-eGFP mouse cervical spinal cord section


     

Tyrosine Hydroxylase staining reveals sympathetic nerve in a mouse lung

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X


Blood vessel staining of a mouse heart

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X


Nerve staining of a mouse kidney

Cleared and labeled with our pipeline, imaged with LS18 at 3.2X



Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse heart

Cleared and labeled with our pipeline, imaged with LS18 at 3.2x.



Endogenous Tie2- tdTomato visualized throughout an entire adult mouse brain

Cleared with our pipeline, imaged with LS18 at 4x.


Mesenchymal stem cells(green)and stromal cells(red)in a mouse femur.

Cleared with PEGASOS, imaged with LS18 at 6.3x.


Endogenous Tie2- tdTomato visualized in a mouse kidney.

Cleared with our pipeline, imaged with LS18 at 12.6X.

Endogenous Thy1-driven eGFP expression in a mouse brain

Cleared with our pipeline, imaged with LS18 at 6.3x.

                     

Endogenous Thy1-driven eGFP expression visualized throughout an entire adult mouse brain

Cleared with PEGASOS, imaged with LS18 at 6.3x.


Endogenous Tie2- tdTomato visualized throughout a mouse testis.

Cleared with our pipeline, imaged with LS18 at 6.3x.


Endogenous Thy1-driven eGFP expression visualized throughout an entire adult mouse brain.

Cleared with PEGASOS, imaged with LS18 at 6.3x.


Endogenous Thy1-driven eGFP expression visualized in an adult mouse brain.

Cleared with PEGASOS, imaged with LS18 at 6.3x.


Visualizing one side hippocampus of a mouse brain with Endogenous Thy1-driven eGFP expression

Cleared with PEGASOS, imaged with LS18 at 6.3x.


Sub sample volume within a Thy1-eGFP mouse brain

Cleared with CUBIC, imaged with LS18 at 20x.


Glial cells visualizing in an adult mouse brain

Cleared with SHIELD, imaged with LS18 at 6.3x.

 

Mouse brain stained with a lectin dye labeling blood vessels

Cleared and labeled with SHIELD, imaged with LS18 at 6.3x.


Endogenous Tie2- tdTomato expression visualized in an adult mouse brain

Cleared with our pipeline, imaged with LS18 at 20x.



CST(Corticospinal tract)in a mouse brainstem

Cleared with uDISCO, imaged with LS18 at 6.3x.


NeuN staining in an intact mouse heart

Cleared and labeled with our pipeline, imaged with LS18 at 4x.


PI staining nucleus in an intact mouse heart

Cleared and labeled with CUBIC, imaged with LS18 at 6.3x.


Endogenous VGluT2- tdTomato expression visualized in a mouse lung

Cleared with SHIELD, imaged with LS18 at 6.3x.


Endogenous Tie2- tdTomato expression visualized in a mouse liver

Cleared with our pipeline, imaged with LS18 at 4x.


Tyrosine Hydroxylase staining reveals sympathetic nerve in an intact mouse spleen

Cleared and labeled with our pipeline, imaged with LS18 at 6.3x



Endogenous Tie2- tdTomato expression visualized in a mouse stomach

Cleared with PEGASOS, imaged with LS18 at 5x.


Folliclesredand nucleusblue)staining in a mouse ovary.

Cleared with ScaleS, imaged with LS18 at 12.6x.


Endogenous VGluT2-tdTomato visualized throughout an entire mouse spine.

Cleared with PEGASOS, imaged with LS18 at 4x.


Endogenous VGluT2-tdTomato visualized in a mouse hindleg bone

Cleared with PEGASOS, imaged with LS18 at 4x.


CD146red)and Krt14(green)staining reveals blood vessels and basal cells in human mammary gland(left: cancer tissue; right: normal tissue)

Cleared and labeled with Adipo-Clear method, imaged with LS18 at 5x.



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