LS18 tiling light sheet microscope

Based on the virtual tiling light sheet technology, Nuohai LS18 tiling light sheet microscope breaks the conflict among 3D spatial resolution, SNR and visual field in traditional light sheet microscope and provides 3D imaging solution at higher resolution.  The real-time light sheet adjustment and calibration function based on the tiling technology ensures the stability and ease-of-use in various application. Furthermore, LS18 provides component and process optimization solutions for various samples to facilitate the users in getting multi-signal tissue structural information at μm, sub-μm and hundreds-of-nm level resolution by adjusting the resolution, speed, magnification parameters and the sample processing methods. The 3D image analysis software and data analysis program facilitate the users in result analysis and interpretation at higher efficiency. LS18 is thus widely used in the fundamental life science fields such as developmental biology, oncology and neuroscience, and pharmaceutical fields including drug development, pharmacodynamic evaluation and histopathology diagnosis. 

锘海LS18平铺光片显微镜成像展示：

C57整鼠

经锘海组织透明化试剂盒处理，使用LS18平铺光片显微镜进行整体3D成像，可清晰观察小鼠全身组织分布和形态，

样本尺寸：37.3mm*77.4mm*23.1mm，

小鼠头部细节

小鼠胸部细节

Thy1-GFP成年小鼠完整中枢神经系统

采用锘海亲水型透明化试剂盒制样，分辨率：2μm×2μm×5μm

Thy1-eGFP小鼠完整颅骨脑样本

采用锘海透明化试剂盒，使用锘海LS18平铺光片显微镜进行三维成像

神经细节展示

 小鼠全脑血管成像                                             小鼠肺成像                           小鼠肾脏血管成像   

     乳腺肿瘤成像                       小鼠骨双色成像                胎鼠卵巢双色成像              小鼠睾丸血管成像

   小鼠脾脏神经成像                   小鼠胚胎成像                     小鼠肠血管成像                   小鼠胃血管成像

已安装锘海LS18平铺光片显微镜机构：

LS18用户已发文章：

2024

Neuron

Gut-induced alpha-Synuclein and Taupropagation initiate Parkinson’s and Alzheimer’s disease co-pathology and behavior impairments

作者：Jie Xiang,Jingrong Tang, Fei Kang, Jiajun Ye, Yueying Cui, Zhentao Zhang, Jing Wang, ShengxiWu, and Keqiang Ye

American Journal of Physiology-Endocrinology and Metabolism

Characterization of a novel adipose tissuelocated between abdominal lymph nodes and cervix/prostate in mice

作者：Lihao Hu, GaochenZhang, Xiaoyu Tong, Lulu Wang, Xiang Qiu, Hongbo Yang, Xinmei Liu, and HefengHuang

Research Square

Astrocytic Bestrophin1 in the AnteriorCingulate Cortex Modulates the Formation and Persistence of Morphine AddictionMemory

作者：Linlin Sun,Zepeng Liu, Yifan Li, Xiaorui Lin, Yu Huo, Shengyang Guo, Xiang Fan, Xinzhi Ye,Yun Yao, Jiao Liu, Ning Wang, Sen Song, and Yijing Li

Science China Life Science

Cellular atlases of ovarianmicroenvironment alterations by diet and genetically-induced obesity

作者：YonghuiJiang, Xueying Gao, Yue Liu, Xueqi Yan, Huangcong Shi,Rusong Zhao, Zi-JiangChen, Fei Gao, Han Zhao & Shigang Zhao

Nature Neuroscience

Somatosensory cortex and central amygdalaregulate neuropathic pain-mediated peripheral immune response via vagal projectionsto the spleen

作者：Xia Zhu,Ji-Ye Huang, Wan-Ying Dong, Hao-Di Tang, Si Xu, Qielan Wu, Huimin Zhang,Ping-Kai Cheng, Yuxin Jin, Meng-Yu Zhu, Wan Zhao, Yu Mao, Haitao Wang, YanZhang, Hao Wang, Wenjuan Tao , Yanghua Tian , Li Bai & Zhi Zhang

Science of The Total Environment

Kidney injury contributes to edema ofzebrafish larvae caused by quantum dots

作者：LuanjinWang , Cixin Liang , Naying Zheng , Chunyan Yang , Sen Yan , Xiang Wang ,Zhenghong Zuo, Chengyong He

2023

Research

Characteristic Features of Deep Brain Lymphatic Vessels and Their Regulation by Chronic Stress

作者：Junzhuang Chang, Bingqing Guo, Yan Gao, Wei Li, Xiaoyu Tong, Yi Feng, and Nashat Abumaria

Nature Metabolism

Thermogenic adipocyte-derived zinc promotes sympathetic innervation in male mice

作者：Junkun Jiang, Donglei Zhou, Anke Zhang, Wenjing Yu, Lei Du Huiwen Yuan, Chuan Zhang, Zelin Wang, Xuyang Jia Zhen-Ning Zhang & Bing Luant

Journal of Experimental & Clinical Cancer Research

Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinoma

作者：Lingyun Zhou, Linye He, Chang?Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin, Kunlin Xie, Javier Ampuero, Zhihui Li, Duoduo Lv, Miao Liu, Manuel Romero?Gómez, Yiguo Hu and Hong Tang

Microbiology Spectrum

Spatiotemporal observations of host-pathogen interactions in mucosa during SARS-CoV-2 infection indicate a protective role of ILC2s

作者：Wei Hu, Lu Meng, Chao Wang, Wenhan Lu, Xiaoyu Tong, Rui Lin, Tao Xu, Liang Chen, An Cui, Xiaoqing Xu, Anni Li, Jia Tang, Hongru Gao, Zhenle Pei, Ruonan Zhang, Yicong Wang, Yu Wang,Wendong Han,Ning Jiang,Chenglong Xiong,Yi Feng, Kuinyu Lee, Mingquan Chen

EMBO Reports

Role and mechanism of PVN–sympathetic–adipose circuit in depression and insulin resistance induced by chronic stress 

作者：Jing Wang, Linshan Sun, Jingjing You, Honghai Peng, Haijing Yan, Jiangong WangFengjiao Sun, Minghu Cui, Sanwang Wang, Zheng Zhang, Xueli Fan, Dunjiang Liu, Cuilan Liu, Changyun Qiu, Chao Chen, Zhicheng Xu, Jinbo Chen, Wei Li,& Bin Liu

Science

3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing

作者：Xiaoyan Lyu , Feiyun Cui Hang Zhou , Bo Cao , Xiaolan Zhang , Mi

Human Brain

Study on the transparent dFC-Tesos microscopic imaging method for large tissues in human brain

作者：Di-Di Li, Xue Wang, Wen-Ying Qiu, Jing-Li Yang, Wei-Ping Wang, Xiao-Liang Wang

Nature Materials

Rapid fabrication of physically robust hydrogels

作者：Bingkun Bao, Qingmei Zeng, Kai Li, Jianfeng Wen, Yiqing Zhang, Yongjun Zheng, Renjie Zhou, Chutong Shi, Ting Chen, Chaonan Xiao, Baihang Chen, Tao Wang, Kang Yu, Yuan Sun, Qiuning Lin , Yong He, Shantung Tu & Linyong Zhu

Neuroscience Bulletin 

Spatiotemporal Dynamics of the Molecular Expression Pattern and Intercellular Interactions in the Glial Scar Response to Spinal Cord Injury

Leilei Gong ,Yun Gu ,Xiaoxiao Han · Chengcheng Luan · Chang Liu · Xinghui Wang · Yufeng Sun · Mengru Zheng · Mengya Fang· Shuhai Yang · Lai Xu · Hualin Sun · Bin Yu · Xiaosong Gu · Songlin Zhou

Cell Proliferation

Single-cell RNA sequencing reveals new subtypes of lens superficial tissue in humans

作者：Meng-Chao Zhu,Wei Hu,Lei Lin Qing-Wen Yang,Lu Zhang,Jia-Lin Xu,Yi-Tong Xu, Jia-Sheng Liu,Meng-Di Zhang,Xiao-Yu Tong,Kai-Yi Zhu,Ke Feng,Yi Feng, Jian-Zhong Su,Xiu-Feng Huang,Jin Li,

The American Journal of Pathology

Defective Uterine Spiral Artery Remodeling and Placental Senescence in a Pregnant Rat Model of Polycystic Ovary Syndrome

作者：Min Hu, Yuehui Zhang, Xu Zhang, XiuYing Zhang, Xinyue Huang, Yaxing Lu, Yijia Li, Mats Br?nnstr?m,Amanda Nancy Sferruzzi-Perri, Linus . Shao, and H?kan Billigy

Cell Journal

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats after Tissue Clearing

作者：Huaixuan Sheng, , Mingru Huang, , Huizhu Li, , Luyi Sun, , Sijia Feng,, Xiner Du, Yicong Wang, Ph, Xiaoyu Tong, M.Sc., Yi Feng, M.D., Ph.D., Jun Chen, Ph.D. , Yunxia Li, M.D.

Cerebral Cortex

Three-dimensional topography of eye-specific domains in the lateral geniculate nucleus of pigmented and albino rats

作者：Hangqi Li, Qiuying Zhou, Yanlu Chen, Huijie Hu, Liang Gao, Toru Takahata

2022

Cell Reports

Bacillus subtilis programs thedifferentiation of intestinal secretory lineages to inhibit Salmonellainfection

作者：QihangHou, Junpeng Jia, Jian Lin, Linda Zhu, Shuang Xie, Qinghua Yu, Yuchen Li

Frontiers in Neurology

Knockdown of NRSF Alleviates Ischemic BrainInjury and Microvasculature Defects in Diabetic MCAO Mice

作者：Cheng-FengHe , Wen-Jiao Xue , Xiao-Die Xu , Jian-Tao Wang , Xin-Ru Wang ,Yi Feng,Hou-Guang Zhou and Jing-Chun Guo

Nature

Olfactory sensory experience regulatesgliomagenesis via neuronal IGF1

作者：PengxiangChen, Wei Wang, Rui Liu, Jiahui Lyu,Lei Zhang, Baizhou Li, Biying Qiu, AnhaoTian, Wenhong Jiang, Honggang Ying, Rui Jing, Qianqian Wang, Keqing Zhu,Ruiliang Bai, Linghui Zeng, Shumin Duan& Chong Liu

Nature Communications

Carbonized paramagnetic complexes of Mn(II) as contrast agents for precise magnetic resonance imaging ofsub-millimeter-sized orthotopic tumors

作者：RuixueQin, Shi Li, Yuwei Qiu, Yushuo Feng, Yaqing Liu, Dandan Ding, Lihua Xu,Xiaoqian Ma, Wenjing Sun& Hongmin Chen

Chinese Medicine

Three-dimensional visualization ofelectroacupuncture-induced activation of brown adipose tissue via sympatheticinnervation in PCOS rats

作者：HongruGao, Xiaoyu Tong, Wei Hu, Yicong Wang, Kuinyu Lee, Xiaoqing Xu, Jiemei Shi,Zhenle Pei, Wenhan Lu, Yuning Chen, Ruonan Zhang, Zheyi Wang, Ziyu Wang,Chengzhi Han, Yu Wang and Yi Feng

Neuroscience Bulletin

Spatiotemporal Dynamics of the MolecularExpression Pattern and Intercellular Interactions in the Glial Scar Response toSpinal Cord Injury

作者：Gong L, Gu Y, HanX, Luan C, Liu C, Wang X, Sun Y, Zheng M, Fang M, Yang S, Xu L, Sun H, Yu B, GuX, Zhou S

2021

Science

Proliferation tracing reveals regional hepatocyte generation in liver homeostasis and repair

作者：Lingjuan He, Wenjuan Pu, Xiuxiu Liu, Zhenqian Zhang, Maoying Han, Yi Li, Xiuzhen Huang, Ximeng Han, Yan Li, Kuo Liu, Mengyang Shi, Liang Lai, Ruilin Sun, Qing-Dong Wang, Yong Ji, Jan S. Tchorz, Bin Zhou

Nanoscale

Concentrated small extracellular vesicles from menstrual blood-derived stromal cells improve intrauterine adhesion, a pre-clinical study in a rat model

作者：Siwen Zhang, Qiyuan Chang, Pingping Li, Xiaoyu Tong, Yi Feng, Xinyao Hao, Xudong Zhang, Zhengwei Yuane and Jichun Tan

STAR Protocols

Protocol for constructing a versatile tiling light sheet microscope for imaging cleared tissues

作者：Ruili Feng, Dongyue Wang, Yanlu Chen, Jing Lu, and Liang Gao

2020

Cell Reports  

A Versatile Tiling Light Sheet Microscope for Cleared Tissues Imaging

作者：Yanlu Chen, Xiaoliang, Dongdong Zhang, Chunhui Wang, Ruili Feng, Xuzhao Yao Wen,1,2,12 Hao Xu, Xinyi Shirley Zhang,6 Xiao Yang, Yongyi Chen, Yi Feng, Bo Zhou, Bi-Chang Chen,Kai Lei, Shang Cai, Jie-Min Jia and Liang Gao

中国光学期刊

光片荧光显微成像技术及应用进展

作者：于湘华，刘超，柏晨，杨廷龙，彭彤，但且，闵俊伟，姚保利

锘海LS18平铺光片显微镜成像展示：

神经细节展示

主要参数：

• 针对课题组实验需求，提供组织透明化、染色及LS18平铺光片显微镜线上、线下介绍报告