Product description:

Theproduct immobilizes biomacromolecules to a hydro-gel. Tissue expansion isfurther achieved through isotropic expansion of the hydro-gel in water, whichimproves imaging resolution with the same imaging method.

Productfeatures:

1.The product can be used forexpansion of both intact and sectioned tissues.

2. The expanded tissues aresuitable for imaging with common microscopes, such as light sheet microscopeand confocal microscope, etc..

3.The product has a high tissue expansion efficiency. For instance, the expansionof a whole adult mouse brain only takes 9-10 days.

4. Theproduct can be used to expand tissueswith and without endogenous fluorophores. Genetically encoded fluorophorescan be preserved with high efficiency after expansion. Fluorescent staining canbeapplied before delipidation, after delipidation, or after expansion followingthe instruction.

5.The expanded tissue has a high mechanical strength and preserve the cellularand subcellular structures effectively.

6. Theexpansion ratiocan be adjusted between 3 and 4 following the instruction.

7.The productuses low toxicity chemicals for tissue expansion.

The expansion process of a mousebrain

The expansion of various mouse organs

Imaging Cases:

                                                                    Thy1-GFP mouse brain neuron

                                                                    4X expansion

                                                                              Resolution：0.5×0.5×1.25μm3

                                                                    Thy1-GFP mouse brain neuron 

                                                                    4X expansion

                                                                    Resolution：70×70×200 nm3

                                                             Green:ChAT-GFPmouse spinal cord motor neuron

                                                             Purple:PV-tdTomatomouse sensory neuron

                                                             4X expansion

                                                            Resolution：100×100×250 nm3

Experimental operation video:

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