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LS Microscopes Accessories

Date:2019/5/16 14:05:40


Objective Lenses

The LS Microscope (Lumascope) product line uses infinity corrected, Royal Microscopy Society (RMS) threaded objectives with a 45 mm parfocal distance. We offer a family of objectives that provides a range of price-performance (see Objectives Offered Table below). You may also be able to use your own objectives, including those typically used in upright microscopes. If RMS threaded, they can be installed directly, or they may work with an objective adapter (inquire at Etaluma). However, they should always be infinity-corrected in order to achieve high resolution.


Coverglass Correction

Objectives are corrected for different thicknesses of material between the objective and sample (referred to as cover glass thickness or CGT) and thus should be considered when choosing objectives. If the material is a coverslip or bottom of a glass bottom microplate, the thickness is 0.17 mm. If a microscope slide or plastic microplate well bottom, the thickness will likely be 1 mm but should always be confirmed. The material thickness correction (often referred to as coverglass correction) is typically shown on the objective body and listed for each objective in the Objectives Offered Table below. It may also be possible to adjust the material thickness in order to use a certain objective, e.g., by inverting a microscope slide with mounted coverslip so that a higher magnification objective corrected for 0.17 mm can be used, or by switching to a glass bottom microplate from a plastic bottom microplate.


Phase Contrast

Phase contrast objectives have a phase plate with a ring shaped phase shifter at the rear focal plane. This ring is matched to a ring annulus at the front focal plane of the phase condenser. With Etaluma’s Phase Contrast Accessory, the appropriate annulus (mask) is contained within a slider. The slider also contains an open position (no mask) so that phase objectives can be used for fluorescence and brightfield as well.



Fluorescence and Brightfield

The objectives optimal for your application depend on your samples, labware and resolution desired. In general, the better the objective, the better the resolution. With regard to fluorescence, high resolution images can be obtained using the Meiji Plan Apochromat objectives. However, the Olympus collared objectives are the most versatile because the cover glass thickness (CGT) correction can be adjusted from 0 mm to 2 mm, allowing imaging through a full range of labware including coverslips, slides, glass and plastic bottom slides, flasks, and Petri dishes.

If you do not see objectives below that meet your needs, inquire at Etaluma. For example, some objectives are available in long working distance (LWD) versions (typically with an increase in price). These may be denoted “TC” for tissue culture, indicating they are appropriate for most labware thicknesses.

We do not recommend using very inexpensive objectives because they can auto-fluoresce at lower wavelengths, adding significantly to image background.

 

Use of Oil Immersion

As with most 100x objectives. Microscopy oil increases the numerical aperature (light collection efficiency) by matching the refractive index of the lens and eliminating interfacial losses.Oil immersion objectives work well on LS Microscopes. Because 100x objectives typically have a coverslip correction of 0.17 mm, microscope slides should be inverted so the coverslip is toward the objective.


Phase Contrast

Phase contrast objectives have a phase plate with a ring shaped phase shifter at the rear focal plane. This ring is matched to a ring annulus at the front focal plane of the phase condenser. With Etaluma’s Phase Contrast Accessory, the appropriate annulus (mask) is contained within a slider. The slider also contains an open position (no mask) so that phase objectives can be used for fluorescence and brightfield as well.




About Nuohai Life Science

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