How does your phase imaging measure up?
Many phase contrast imaging and analysis techniques claim to be quantitative, but may use indirect or proxy measurements to define and quantify cells.
Quantitative Phase Imaging (QPI) generates high contrast, high quality images from which direct measures of morphology and motility can be extracted, allowing comprehensive analysis at both population and individual cell level.
Achieve a greater level of scrutiny with QPI
* High resolution imaging defines EVERY cell to give a true measure of cell count.
* DIRECT measurement of cell metrics.. Suitable for heterogeneous populations where smaller sub populations can be differentiated.
* Computational technique. Calculates the extent of phase shift allowing thickness dependent measures to be determined for each cell
e.g. cell volume, dry cell mass and sphericity.
* Insufficient resolution to define each cell. Confluence used to ESTIMATE cell count.
* Analysis based on INDIRECT population level metrics, i.e.confluency, which characterises the cell population as homogeneous.
* Optical technique.
Unable to assess the extent of phase shift so individual cell parameters cannot be determined.
QPI - made to measure imaging